Deleterious effects of endotoxin on cultured endothelial cells: An in vitro model of vascular injury
- 1 June 1981
- journal article
- research article
- Published by Springer Nature in Inflammation
- Vol. 5 (2), 115-126
- https://doi.org/10.1007/bf00914201
Abstract
The effects of endotoxin-triggered granulocytes on the viability of endothelial cells in vitro was investigated. Endotoxin or its lipid A component caused granulocytes to adhere to and significantly damage cultured endothelial cells. Fresh serum is not necessary but does amplify both adherence and endothelial injury. Much of the endothelial injury was inhibited by free-radical scavengers or by blocking granulocyte adhesion to endothelial cells and appears to result from free radical production by the stimulated granulocyte. Studies in this model suggest a pathogenic role for the endotoxin-triggered granulocyte in the Shwartzman reaction and perhaps related clinical disorders.This publication has 35 references indexed in Scilit:
- Macrophage stimulation by bacterial lipopolysaccharides. II. Evidence for differentiation signals delivered by lipid A and by a protein rich fraction of lipopolysaccharides.The Journal of Experimental Medicine, 1978
- Oxygen radicals mediate endothelial cell damage by complement-stimulated granulocytes. An in vitro model of immune vascular damage.JCI Insight, 1978
- Structural features of Salmonella typhimurium lipopolysaccharide required for activation of tissue factor in human mononuclear cells.JCI Insight, 1977
- Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis.JCI Insight, 1975
- Increased Sheep Lung Vascular Permeability Caused by Pseudomonas BacteremiaJCI Insight, 1974
- Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIAJCI Insight, 1973
- Evidence for Participation of Granulocytes in the Pathogenesis of the Generalized Shwartzman Reaction: A ReviewThe Journal of Infectious Diseases, 1973
- THE GRANULOCYTOPENIC RESPONSE IN HEMORRHAGIC SHOCK*JCI Insight, 1959
- STUDIES ON THE GENERALIZED SHWARTZMAN REACTIONThe Journal of Experimental Medicine, 1953
- STUDIES ON THE MECHANISM OF THE SHWARTZMAN PHENOMENONThe Journal of Experimental Medicine, 1951