The isolation of nuclei from animal cells in culture
- 20 November 1962
- journal article
- research article
- Published by The Royal Society in Proceedings of the Royal Society of London. B. Biological Sciences
- Vol. 156 (965), 521-523
- https://doi.org/10.1098/rspb.1962.0053
Abstract
The following method permits the isolation of nuclei from HeLa cells. Virtually all the nuclei in a population of cells can be recovered and the isolated nuclei are free from adherent cytoplasm. Examination of nuclei isolated from cells which had been incubated in media containing [$^{14}$C] thymidine and [$^{14}$C] adenine indicated that not more than a few per cent of the DNA or of the labelled nuclear RNA were lost during the isolation procedure.This publication has 3 references indexed in Scilit:
- The relationship between nuclear and cytoplasmic ribonucleic acidProceedings of the Royal Society of London. B. Biological Sciences, 1962
- Interference Microscopy and Mass DeterminationNature, 1952