Inhibition of Glyceraldehyde-3-Phosphate Dehydrogenase by Follicle-Stimulating Hormone Preparations In Vitro

Abstract

Rabbit muscle GAPDH (E.C.1.2.1.12) was inhibited by two preparations of FSH, one ovine and the other porcine. A third preparation, also of ovine origin, did not inhibit this enzyme. This suggested that the inhibition was not due to FSH but a contaminant. This inhibition of glyceraldehyde-3-phosphate dehydrogenase was specific. None of the other pituitary hormones had any effect on this enzyme and other NAD-linked and NADP-linked dehydrogenases were not inhibited by these FSH preparations. Abbreviations used ADH = alcohol dehydrogenase EDTA= disodium ethylenedinitrilotetracetate FSH = follicle-stimulating hormone GDH = L-glutamic dehydrogenase G6PDH = glucose-6-phosphate dehydrogenase GAPDH = glyceraldehyde-3-phosphate dehydrogenase LH = luteinizing hormone α-MSH = α-melanocyte-stimulating hormone 6PGDH = 6-phosphogluconate dehydrogenase STH = growth hormone TSH = thyroid-stimulating hormone