Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose
- 13 February 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 244 (1), 127-131
- https://doi.org/10.1016/0014-5793(89)81177-9
Abstract
Endoglucanase CenA of Cellulomonas fimi comprises an N-terminal cellulose-binding domain and a C-terminal catalytic domain joined together by a sequence of 23 proline and threonine residues (the Pro-Thr box). The domains function independently when separated by proteolysis. TnphoA has been used to generate cenA′-′phoA fusions. CenA′-′PhoA fusion polypeptides which contain the entire cellulose-binding domain of CenA bind to cellulose, allowing their purification from periplasmic extracts in a single, facile step. This result has implications for purification or immobilisation of chimeric proteins on a cheap cellulose matrixKeywords
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