Cluster of point mutations predetermined by a quasipalindromic nucleotide sequence in plasmid pBR322 DNA
- 12 February 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 261 (1), 28-30
- https://doi.org/10.1016/0014-5793(90)80628-v
Abstract
Development of a cluster of point mutations due to the correction of an imperfect hairpin in plasmid DNA was investigated. Plasmid pBR322 DNA containing opposite double-strand DNA lesions in the region of a quasipalindrome was constructed. For this aim plasmid DNA was cleaved at the BamHI site, and cytosine residues of the sticky ends were modified by O-methylhydroxylamine. Modified linearized plasmid DNA was ligated and used for transformation of E.coli cells. Tetracycline-sensitive transformants were selected, and the mutants were characterized by restriction and sequencing analysis. One mutant contained a cluster of point mutations. The distribution of mutations was consistent with the cluster having arisen through correction of the imperfect hairpin formed by the quasipalindromeKeywords
This publication has 14 references indexed in Scilit:
- Highly selective chemical modification of cruciform loops by diethyl pyrocarbonateNucleic Acids Research, 1986
- Demonstration of the production of frameshift and base-substitution mutations by quasipalindromic DNA sequences.Proceedings of the National Academy of Sciences, 1984
- Structural intermediates of deletion mutagenesis: a role for palindromic DNA.Proceedings of the National Academy of Sciences, 1984
- Perfect palindromic lac operator DNA sequence exists as a stable cruciform structure in supercoiled DNA in vitro but not in vivo.Proceedings of the National Academy of Sciences, 1983
- Unique Self-complementarity of Palindromic Sequences Provides DNA Structural Intermediates for MutationCold Spring Harbor Symposia on Quantitative Biology, 1983
- Model for the participation of quasi-palindromic DNA sequences in frameshift mutation.Proceedings of the National Academy of Sciences, 1982
- Nucleotide sequence of the region of the origin of replication of the broad host range plasmid RK2Molecular Genetics and Genomics, 1981
- Gene-directed mutagenesis in bacteriophage T7 provided by polyalkylating RNAs complementary to selected DNA sites.Proceedings of the National Academy of Sciences, 1980
- Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigensCell, 1979
- Nucleotide sequence of a region of human mitochondrial DNA containing the precisely identified origin of replicationNature, 1979