Stabilization of Catalase-Ammonia Complex in Frozen Solution.

Abstract

It is demonstrated that the ammonia complex of catalase from Micrococcus lysodeikticus is difficult to detect in liquid solution but easy to study in frozen solution. Electron spin resonance and light absorption of catalase and the ammonia complex have been measured in frozen solutions down to 77TC. Factors that possibly could effect a stabilization of the ammonia complex in the matrix of ice are discussed.