Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.
- 15 September 1991
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (18), 7978-7982
- https://doi.org/10.1073/pnas.88.18.7978
Abstract
A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.Keywords
This publication has 23 references indexed in Scilit:
- Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning.Proceedings of the National Academy of Sciences, 1991
- Human and mouse monoclonal antibodies by repertoire cloningTrends in Biotechnology, 1991
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- Searching for Peptide Ligands with an Epitope LibraryScience, 1990
- Hormone phage: An enrichment method for variant proteins with altered binding propertiesProteins-Structure Function and Bioinformatics, 1990
- Gene-III protein of filamentous phages: Evidence for a carboxyl-terminal domain with a role in morphogenesisVirology, 1984
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983
- Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.Proceedings of the National Academy of Sciences, 1983
- Effects of bacteriophage f1 gene III protein on the host cell membraneMolecular Genetics and Genomics, 1982
- Membrane biogenesis: cotranslational integration of the bacteriophage f1 coat protein into an Escherichia coli membrane fraction.Proceedings of the National Academy of Sciences of the United States of America, 1979