Processing of interleukin‐1 in cells of monocytic lineage is differentiation‐dependent
- 1 April 1992
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 205 (1), 295-301
- https://doi.org/10.1111/j.1432-1033.1992.tb16780.x
Abstract
The interleukin-1 (IL-1) alpha and beta precursor proteins are processed and released from several cell types in the absence of a canonical signal peptide. To gain some insight into the mechanisms that allow the production of IL-1 alpha and beta, we have investigated by immunoprecipitation the synthesis, their release and processing in a promyeloblastic cell line of tumoral origin, U937, and in peripheral blood monocytes. We show that U937 monocytic cells, on induction with a tumor-promoting agent, synthesize and release into the culture medium proIL-1 beta but do not process it. Similarly, peripheral blood monocytes left in adherence for 24 h or longer, prior to addition of lipopolysaccharide, synthesize and release proIL-1 alpha and beta without detectable processing of either cytokine. Processing and release of IL-1 alpha and beta by peripheral blood monocytes can be observed when monocytes are left to adhere for periods less than 15 h before lipopolysaccharide addition. IL-1 alpha and beta show similar kinetics of release from the cells, suggesting the existence of a common mechanism regulating their secretion. Since peripheral blood monocytes left in adherence in the presence of lipopolysaccharide differentiate into macrophages, we conclude that release and processing of IL-1 can occur independently and that processing depends on the stage of differentiation of monocytes, i.e. only the monocytes at an early stage of differentiation produce 17-kDa IL-1 alpha and beta.Keywords
This publication has 47 references indexed in Scilit:
- The processing of interleukin-1β studied with antibodies raised against synthetic peptides from the precursor N-terminal regionCytokine, 1991
- Insertion of an elastase-binding loop into interleukin-1βProtein Engineering, Design and Selection, 1991
- Structure and function of membrane IL-1Molecular Immunology, 1988
- Human interleukin 1 beta is not secreted from hamster fibroblasts when expressed constitutively from a transfected cDNA.The Journal of cell biology, 1988
- Interleukin 1: more than a mediator between leukocytesTrends in Pharmacological Sciences, 1988
- Immunocytochemical detection of interleukin 1 within stimulated human monocytes.The Journal of Experimental Medicine, 1986
- There is more than one interleukin 1Immunology Today, 1986
- Normal human alveolar macrophages obtained by bronchoalveolar lavage have a limited capacity to release interleukin-1.JCI Insight, 1984
- In vitro activation of a human macrophage-like cell lineNature, 1979
- Establishment and characterization of a human histiocytic lymphoma cell line (U‐937)International Journal of Cancer, 1976