Evaluation of the automicrobic system for detection of resistance of Staphylococcus aureus to methicillin
- 1 April 1984
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 19 (4), 464-467
- https://doi.org/10.1128/jcm.19.4.464-467.1984
Abstract
The AutoMicrobic system (AMS) was tested for its ability to determine oxacillin and gentamicin susceptibility of 98 known oxacillin-susceptible and 103 known oxacillin-resistant S. aureus isolates. AMS and reference oxacillin susceptibility results were in agreement for all 95 (100%) oxacillin-susceptible isolates. Only 23 (22.3%) of the 103 known oxacillin-resistant isolates were correctly reported. For the known oxacillin-resistant isolates, 65 received AMS reports at 3-4 h, with only 9% being correct; 38 were reported at 5-6 h, with 47% being correct. The reliability of AMS gentamicin susceptibility results was evaluated by testing the 198 S. aureus isolates in parallel with MIC-2000 broth dilution tests. AMS gentamicin susceptibility results were reliable and essentially identical to MIC-2000 results. The possibility of improving AMS oxacillin resistance detection by using gentamicin resistance as a linked screening marker for oxacillin resistance was evaluated with data from the parallel AMS and MIC-2000 gentamicin susceptibility tests and from data accrued on recent clinical laboratory isolates. By these 2 approaches, respective sensitivities of 97 and 99.8%, and specificity of 72%, were found for detection of oxacillin-resistant isolates by using gentamicin resistance as a marker.This publication has 26 references indexed in Scilit:
- Comparison of Automated and Rapid Manual Methods for the Same-day Identification of EnterobacteriaceaeAmerican Journal of Clinical Pathology, 1983
- Clinical evaluation of new AMS GSC Plus CardDiagnostic Microbiology and Infectious Disease, 1983
- Epidemic neonatal gentamicin-methicillin-resistant Staphylococcus aureus infection associated with nonspecific topical use of genetamicinThe Journal of Pediatrics, 1980
- Methicillin-Resistant Staphylococcus aureus: Introduction and Spread Within a HospitalAnnals of Internal Medicine, 1980
- The Automicrobic System for urinesJournal of Clinical Microbiology, 1979
- Gentamicin-resistant Staphylococcus aureus. Emergence in an intensive care nursery.1979
- Methicillin-Resistant Staphylococcus aureus Susceptibility Testing by an Automated System, Autobac IAntimicrobial Agents and Chemotherapy, 1978
- Infections due to gentamicin-resistant Staphylococcus aureus strain in a nursery for neonatal infantsAntimicrobial Agents and Chemotherapy, 1978
- Reliability of the Microdilution Technic for Detection of Methicillin-resistant Strains ofStaphylococcus aureusAmerican Journal of Clinical Pathology, 1977
- OUTBREAK OF HOSPITAL INFECTION WITH A STRAIN OF STAPHYLOCOCCUS AUREUS RESISTANT TO GENTAMICIN AND METHICILLINThe Lancet, 1976