Characterization of the β-lactamase promoter of pBR322
- 1 January 1981
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (11), 2517-2533
- https://doi.org/10.1093/nar/9.11.2517
Abstract
The beta-lactamase promoter of pBR322, derived from Tn3, has been characterized using several techniques. The transcription initiation site is located 35 base pairs from the translation initiation codon of beta-lactamase. The mRNA produced in vitro has a 5' pppGpA terminus. RNA polymerase bound at this start site protects a region from about -50 to +20 from DNase I cleavage using the footprinting technique. RNA polymerase binds rapidly to the beta-lactamase promoter. The half-time of association is less than one-half minute. The half-time of dissociation is approximately 6 hr. A study of the binding of RNA polymerase at different temperatures showed a large change between 11 degrees and 15 degrees C. Comparison of these parameters with those reported for other promoters is discussed.Keywords
This publication has 34 references indexed in Scilit:
- Functional analysis of wild-type and altered tryptophan operon promoters of Salmonella typhimurium in Escherichia coliJournal of Molecular Biology, 1980
- Escherichia coli RNA polymerase and trp repressor interaction with the promoter-operator region of the tryptophan operon of Salmonella typhimuriumJournal of Molecular Biology, 1980
- E. coli RNA polymerase interacts homologously with two different promotersCell, 1980
- Synthesis in E. coli of a polypeptide with human leukocyte interferon activityNature, 1980
- In vitro analysis of the Escherichia coli RNA polymerase interaction with wild-type and mutant lactose promotersJournal of Molecular Biology, 1978
- An expanded transcriptional map of T7 bacteriophage. reading of minor T7 promoter sites in vitro by Escherichia coli RNA polymeraseJournal of Molecular Biology, 1977
- Interaction of RNA Polymerase with Promoters from Bacteriophage fdEuropean Journal of Biochemistry, 1977
- Studies of the binding of Escherichia coli RNA polymerase to DNA: I. The role of sigma subunit in site selectionJournal of Molecular Biology, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Nucleoside Triphosphate Termini from RNA Synthesized in vivo by Escherichia coliScience, 1969