Purification and properties of chlorophyll c from Sargassum flavicans

Abstract

Chlorophyll c from S. flavicans was purified by chromatography on cellulose, silicic acid and aluminum oxide. Purified chlorophyll c was crystallized from aqueous ethanol in the form of hexagonal bipyramids or parallel 4-sided plates. The extinction coefficient, eO 1% 1 cm, of the purest chlorophyll c in acetone at 628-630 m[mu] was 15.8. Absorption maxima were at 628, 580 and 442 mu with relative extinctions at the maxima of 1.0, 0.58-0.73 and 6.9-8.1 respectively. The Mg content of chlorophyll c was 2.31 + 0.045%. The absorption spectrum of chlorophyll c was extremely sensitive to solvents, and to the presence of lipid.