Cloning and domain structure of the mammalian splicing factor U2AF
- 1 February 1992
- journal article
- Published by Springer Nature in Nature
- Vol. 355 (6361), 609-614
- https://doi.org/10.1038/355609a0
Abstract
A complementary DNA clone encoding the large subunit of the essential mammalian pre-messenger RNA splicing component U2 snRNP auxiliary factor (U2AF65) has been isolated and expressed in vitro. It contains two functional domains: a sequence-specific RNA-binding region composed of three ribonucleoprotein-consensus sequence domains, and an arginine/serine-rich motif necessary for splicing but not for binding to pre-mRNA.Keywords
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