Partial characterization of the mRNA that codes for enkephalins in bovine adrenal medulla and human pheochromocytoma.
- 1 January 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (2), 360-364
- https://doi.org/10.1073/pnas.79.2.360
Abstract
[Met]enkephalin and [Leu]enkephalin are derived from a protein in bovine adrenal medulla that contains multiple copies of [Met]enkephalin. Pro-enkephalin mRNA from bovine and human tissue is characterized by use of an oligodeoxynucleotide pentadecamer probe complementary to codons for Tyr-Gly-Gly-Phe-Met ([Met]enkephalin). This probe hybridizes specifically to a species of poly(A)-RNA from adrenal medulla and human pheochromocytoma, (1400-1450 bases), and also to [Met]enkephalin-containing pro-opiomelanocortin mRNA from bovine pituitary (1200 bases) and from mouse pituitary tumor cells (1100 bases). A cloned c[complementary]DNA probe (144 bases) complementary to the region of pro-opiomelanocortin mRNA that codes for lipotropin does not hybridize to the RNA from bovine adrenal medulla, demonstrating that the latter RNA is not pro-opiomelanocortin mRNA. The pentadecamer probe was extended to make cDNA with reverse transcriptase after hybridizing it to adrenal poly(A)-RNA. The sequence of an extended cDNA, 62 bases in length, corresponded exactly to that expected from the amino acid sequence of peptide E (a bovine adrenal peptide containing [Met]- and [Leu]enkephalin sequences). This cDNA also forms a specific hybrid with the RNA from bovine adrenal and human pheochromocytoma, confirming that these species of RNA are pro-enkephalin mRNA.This publication has 26 references indexed in Scilit:
- Biosynthesis in vitro of immunoreactive 31,000-dalton corticotropin/β-endorphin-like material by bovine hypothalamusProceedings of the National Academy of Sciences, 1979
- Chemical synthesis of genes for human insulin.Proceedings of the National Academy of Sciences, 1978
- Enkephalin‐like immunoreactivity in nerve terminals in sympathetic ganglia and adrenal medulla and in adrenal medullary gland cellsActa Physiologica Scandinavica, 1978
- Characterization of a common precursor to corticotropin and beta-lipotropin: identification of beta-lipotropin peptides and their arrangement relative to corticotropin in the precursor synthesized in a cell-free system.Proceedings of the National Academy of Sciences, 1977
- Characterization of a common precursor to corticotropin and beta-lipotropin: cell-free synthesis of the precursor and identification of corticotropin peptides in the molecule.Proceedings of the National Academy of Sciences, 1977
- Common precursor to corticotropins and endorphinsProceedings of the National Academy of Sciences, 1977
- C fragment of lipotropin has a high affinity for brain opiate receptorsNature, 1976
- Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.Proceedings of the National Academy of Sciences, 1976
- Identification of two related pentapeptides from the brain with potent opiate agonist activityNature, 1975
- The base-pairing specificity of cellulose-pdT9Nucleic Acids Research, 1975