Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast.
- 1 December 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (24), 8419-8423
- https://doi.org/10.1073/pnas.82.24.8419
Abstract
Pet56, his3, and ded1 are adjacent but unrelated genes located on chromosome XV of the yeast Saccharomyces cerevisiae. his3 and pet56 are transcribed in opposite directions from initiation sites separated by approximately equal to 200 base pairs. Under normal growth conditions, both genes are transcribed at a similar basal level. Deletion analysis of the his3 gene indicates that the upstream promoter element for constitutive expression is defined by a 17-base-pair region that contains 15 thymidine residues in the coding strand. Sequential deletions of the pet56 gene indicate that this same region is required for wild-type transcription levels. Thus, this poly(dA-dT) sequence acts bidirectionally to activate transcription of two unrelated genes. Transcription of the ded1 gene is initiated approximately equal to 300 base pairs downstream from the his3 gene, and it occurs at a 5-fold higher level. This gene contains a 34-base-pair region containing 28 thymidine residues in the coding strand located upstream from the ded1 TATA box. Deletion of this dA-dT stretch significantly reduces transcription below the wild-type level. Thus, for at least three different yeast genes, naturally occurring stretches of poly(dA-dT) serve as upstream promoter elements for constitutive expression. In addition, it appears that longer stretches of poly(dA-dT) are more effective upstream promoter elements. These transcriptional effects may be due to exclusion of nucleosomes from poly(dA-dT) regions.This publication has 28 references indexed in Scilit:
- A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistanceMolecular Genetics and Genomics, 1984
- Yeast promoters: Positive and negative elementsCell, 1984
- DNA sequences of two yeast promoter-up mutantsNature, 1983
- [12] One-step gene disruption in yeastMethods in Enzymology, 1983
- Promoter mutants of the yeast his3 geneJournal of Molecular Biology, 1981
- Transcription of the his3 gene region in Saccharomyces cerevisiaeJournal of Molecular Biology, 1981
- Sequence-dependent helical periodicity of DNANature, 1981
- Sequence dependence of the helical repeat of DNA in solutionNature, 1981
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridizationCell, 1979