Myristic acid stimulation of bacterial bioluminescence in "aldehyde" mutants.
- 1 January 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (1), 266-269
- https://doi.org/10.1073/pnas.75.1.266
Abstract
The involvement of long chain aldehyde in bacterial luminescence was known both from its being required for light emission in the in vitro reaction with pure luciferase and from its ability to stimulate luminescence in vivo in a certain class of dark aldehyde mutants. The luminescence of some (but not all) of such aldehyde mutants is also stimulated by long chain aliphatic fatty acids, with a marked specificity for myristic (tetradecanoic) acid. This stimulation was demonstrated in aldehyde mutants of 2 spp. of luminous bacteria, Beneckea harveyi and Photobacterium fischeri. The responses, both in intensity and yield, are proportional to the amount of added tetradecanoic acid over a 1000-fold range, down to 10 pmol ml-1. Unsaturated long chain fatty acids are potent inhibitors of the tetradecanoic acid stimulation, but they do not effect the in vivo luminescence of wild-type bacteria.This publication has 19 references indexed in Scilit:
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