Amino acid sequence of the light chain of bovine factor X1 (Stuart factor)

Abstract

The detailed proof of the amino acid sequence of the 140 residues (16,193 daltons) of the L chain of bovine factor X1 (Stuart factor) was presented. Sequence analyses were performed on fragments obtained after chemical cleavage of asparagine-glycine and tryptophanyl peptide bonds and after various enzymatic digestion procedures. Twelve .gamma.-carboxyglutamyl residues were clustered in the amino-terminal 39 residues and 13 half-cystine residues were found in the carboxyl-terminal 91 residues, suggesting 2 domains in the L chain, one exceptionally anionic and the other extensively cross-linked by disulfides.