Physical organization of the ilvEDAC genes of Escherichia coli strain K-12
- 1 January 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (1), 89-93
- https://doi.org/10.1073/pnas.75.1.89
Abstract
The physical location of the ilvEDAC genes on the restriction cleavage map of the ilv region of E. coli K-12 was determined by 2 methods: heteroduplex and endonuclease cleavage analysis of hybrid phages carrying genetically defined parts of the ilv cluster and complementation analysis and enzyme assays to determine ilv gene expression from hybrid plasmids containing DNA restriction fragments of the transducing phage .lambda.h80dilv. The ilvEDA and ilvC operons occupy 2.4 and 0.9 megadalton sequences of DNA, respectively, and are separated by a region of 0.6-0.75 megadalton. The ilvD region, specifying dihydroxy acid dehydrase, has a maximum coding capacity of about 55,000 daltons of polypeptide. Apparently, ilvC is transcribed clockwise on the E. coli K-12 map, in the same direction as ilvEDA. A secondary .lambda. attachment site within ilvC was located on a small (0.45 megadalton) EcoRI fragment. Results are compared to other physical studies of ilv DNA.This publication has 31 references indexed in Scilit:
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