High Performance Liquid Chromatographic Analysis of S-Adenosylmethionine and S-Adenosylhomocysteine in Rat Liver
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 2 (7), 1003-1015
- https://doi.org/10.1080/01483917908060120
Abstract
A sensitive and precise high performance liquid chromatographic method for the simultaneous quantitation of S-adenosylmethionine (SAMe) and S-adenosylhomocysteine (SAH) in rat liver is described. Liver is extracted by homogenization in 1.5 M PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dowex 50 W × 2 and analyzed by HPLC on conventional styrene-type anion exchanger. Analytical chromatography, performed under i-socratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60°C, un der alkaline conditions. The sensitivity of the analytical proce dure allows the determination of SAMe and SAH in less than 200 mg of liver sample.This publication has 7 references indexed in Scilit:
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