The rat insulin‐degrading enzyme
- 15 February 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 317 (3), 250-254
- https://doi.org/10.1016/0014-5793(93)81286-9
Abstract
The primary structure of the rat insulin-degrading enzyme (IDE) was determined by cDNA analysis. Rat IDE, as well as the previously characterized homologs from human and Drosophila, contain the carboxyl-terminal consensus sequence A/S-K-L for peroxisome targeting. A stretch of 43 bp surrounding an alternatively used polyadenylation site is highly conserved between rat and human, suggesting that it may contain important regulatory information. Northern blot analysis revealed two IDE transcripts of 3.7 and 5.5 kb in various tissues. Testis was found to be exceptional in having three different RNAs (3.7, 4.1 and 6.1 kb) at a relatively high abundance. The expression of the IDE gene in testis is correlated with sexual maturation.Keywords
This publication has 19 references indexed in Scilit:
- Molecular characterization of a novel rat protein structurally related to poly(A) binding proteins and the 70K protein of the U1 small nuclear ribonucleoprotein particle (snRNP)Nucleic Acids Research, 1992
- Identification of the metal associated with the insulin degrading enzymeBiochemical and Biophysical Research Communications, 1991
- Atrial natriuretic peptide (ANP) is a high‐affinity substrate for rat insulin‐degrading enzymeEuropean Journal of Biochemistry, 1991
- Degradation of Intraendosomal Insulin by Insulin-Degrading Enzyme Without AcidificationDiabetes, 1991
- Insulin-Degrading Enzyme: Stable Expression of the Human Complementary DNA, Characterization of its Protein Product, and Chromosomal Mapping of the Human and Mouse GenesMolecular Endocrinology, 1990
- An evolutionarily conserved enzyme degrades transforming growth factor-alpha as well as insulin.The Journal of cell biology, 1989
- Human Insulin-Degrading Enzyme Shares Structural and Functional Homologies with E. coli Protease IIIScience, 1988
- Codon usage tabulated from the GenBank Genetic Sequence DataNucleic Acids Research, 1988
- An improved DNA sequencing strategyAnalytical Biochemistry, 1985
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979