CONCANAVALIN A-INDUCED AGGLUTINATION AND BINDING OF CON A TO THE DIFFERENTIATING CELLS OF DICTYOSTELIUM DISCOIDEUM
- 31 December 1975
- journal article
- research article
- Published by Wiley in Development, Growth & Differentiation
- Vol. 18 (3), 311-317
- https://doi.org/10.1111/j.1440-169x.1976.00311.x
Abstract
Changes in agglutinability of D. discoideum cells with Concanavalin A (Con A) during the course of development were investigated. The agglutinability of the cells was assayed under conditions where no spontaneous cell agglutination occurred. There was a progressive decrease in Con A-induced agglutinability during development: a decrease to half from exponentially growing cells to preaggregation cells, and a decrease to a sixth in disaggregated slug cells. Pronase-BAL [2,3 dimercapto-1-propanol] treatment of preaggregation cells did not enhance their agglutinability with Con A. The amounts of sites available for binding Con A were determined with preaggregation and slug cells. Cells were incubated at 4.degree. C and in the presence of NaN3 to avoid possible endocytosis of Con A. No significant differences in numbers of Con A-binding sites per unit area of cell surface was detected among preaggregation cells, those treated with pronase and BAL and cells disaggregated from slugs by similar treatment. It was concluded that the decrease in Con A-induced agglutinability during development is not attributable to changes in the numbers of Con A-binding sites.This publication has 17 references indexed in Scilit:
- Binding of 125I-Concanavalin a and agglutination of embryonic neural retina cells: Age-dependent and experimental changesExperimental Cell Research, 1975
- Cell surface changes during the differentiation of Dictyostelium discoideum: Interaction of cells with Concanavalin AExperimental Cell Research, 1975
- Cell surface changes occurring during sea urchin embryonic development monitored by quantitative agglutination with plant lectinsExperimental Cell Research, 1974
- Unit membrane structural changes following cell association in DictyosteliumExperimental Cell Research, 1973
- DYNAMICS OF ANTIGENIC MEMBRANE SITES RELATING TO CELL AGGREGATION IN DICTYOSTELIUM DISCOIDEUM The Journal of cell biology, 1973
- Lectins: Cell-Agglutinating and Sugar-Specific ProteinsScience, 1972
- Specific inhibition of cell contact formation in Dictyostelium by univalent antibodiesExperimental Cell Research, 1970
- CHANGES IN ELECTRONEGATIVITY OF THE CELL SURFACE DURING THE DEVELOPMENT OF THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM*Development, Growth & Differentiation, 1970
- Disaggregation of slime mold pseudoplasmodia using EDTA and various proteolytic enzymesExperimental Cell Research, 1970
- Microbiological assay of cyclic 3′, 5′-AMPCellular and Molecular Life Sciences, 1970