Chemical synthesis of a self-complementary octanucleotide, dG-G-T-T-A-A-C-C by a modified triester method
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (8), 2809-2824
- https://doi.org/10.1093/nar/5.8.2809
Abstract
The synthesis of a self-complementary octanucleotide, d(G-G-T-T-A-A-C-C-), using a modified triester approach is described. The protected dinucleotides, d(Me2O)TribG(C1C6H4) ibG, d(Me2O)TrT(ClC6H4)T, d(Me2O)TrbzA(ClC6H4)bzA, and d(Me2O)TranC(ClC6H4)anC were synthesized by a one step triester procedure. After removal of the trityl group, the dinucleotides, dT(ClC6H4)T and danC (ClC6H4)anC were coupled to d(Me2O)TribG(ClC6H4)ibG and d(Me2O)TrbzA(ClC6H4)bzA, respectively to yield the respective tetranucleotides. The tetranucleotide, d(Me2O)TrbzA(ClC6H4)bzA(ClC6H4) and (ClC6H4)anC was detritylated and then coupled with d(Me2O)TribG(ClC6H4)ibG(ClC6H4)T(Cl6H4)T to give octanucleotide. The fully protected octanucleotide was deblocked by treatment with aqueous NH3 followed by acid and was characterized by nucleotide sequence analysis.Keywords
This publication has 17 references indexed in Scilit:
- Studies on polynucleotides. 146. High-pressure liquid chromatography in polynucleotide synthesisBiochemistry, 1978
- Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone SomatostatinScience, 1977
- A rapid and convenient synthesis of poly-thymidylic acid by the modified triester approachNucleic Acids Research, 1977
- Duplex formation of a nonionic oligo(deoxythymidylate) analog [heptadeoxythymidylyl-(3'-5')-deoxythymidine heptaethyl ester (d-[Tp(Et)]7T)]with poly(deoxyadenylate). Evaluation of the electrostatic interactionBiochemistry, 1977
- Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides1Nucleic Acids Research, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.Proceedings of the National Academy of Sciences, 1977
- The phosphotriester approach to oligonucleotide synthesis: preparation of oligo- and poly-thymidylic acidsJournal of the Chemical Society, Perkin Transactions 1, 1977
- Synthese von Oligodesoxynucleotiden über PhosphorsäuretriesterEuropean Journal of Inorganic Chemistry, 1969
- Oligonucleotide Synthesis on a Polymer Support1,2Journal of the American Chemical Society, 1965