Reactions of reduced nicotinamide adenine dinucleotide in acid: studies by reversed-phase high-pressure liquid chromatography
- 1 May 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (11), 2234-2238
- https://doi.org/10.1021/bi00604a034
Abstract
Reversed-phase high pressure liquid chromatography was used to isolate acid breakdown products of NADH and products produced when NADH breakdown is catalyzed by glyceraldehyde-3-phosphate dehydrogenase (G-3-PD). Chromatographic and UV spectral data on these and related products support a mechanism for NADH acid degradation involving hydroxy addition at the nicotinamide C-6 followed by cyclization of the ring and the adjacent ribose moiety. G-3-PD catalyzes a reaction in which 2 products are formed which are also intermediates in the acid degradation of NADH (.alpha.- and .beta.-6-hydroxynicotinamide products). Formation of the major acid products fits a 3 step, 1st order mechanism curve, making it possible to calculate the rate constants k2 and k3 as well as the previously determined k1.This publication has 5 references indexed in Scilit:
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