Heterogeneity of Nuclear Populations in Root Meristems
Open Access
- 1 January 1967
- journal article
- research article
- Published by Firenze University Press in Caryologia
- Vol. 20 (1), 87-100
- https://doi.org/10.1080/00087114.1967.10796249
Abstract
Several methods for relating the volumes of individual nuclei to their relative interphase stage have been applied to rapidly proliferating cells of the cortex in root tips of broad bean (Vicia faba). The methods are based upon analysis of the frequency with which particular volume classes appear within a population of nuclei. Presuming random sampling of a particular homogeneous population, it is possible and convenient to relate the volume of an individual nucleus to its age in relative interphase time by using the geometric mean volume and standard deviation of the sampled population to locate a straight line on probability paper. This graph can then be used to estimate the relative interphase time for any given nuclear volume in the population. In cases of heterogeneous populations of nuclei, as found for cells of bean root meristems, synthetically active cells can be distinguished from inactive or quiescent cells by means of isotopic labeling. The individual quiescent cells which occur sporadically throughout the cortex of bean meristems do not incorporate H3-thymidine even after prolonged labeling periods, suggesting a possible relationship with cells of the discrete quiescent center previously described for this and other angiosperm root apices.This publication has 22 references indexed in Scilit:
- DNA synthesis in plant tumor cellsExperimental Cell Research, 1964
- Nucleoprotein changes during the mitotic cycle in Paramecium aureliaExperimental Cell Research, 1961
- NUCLEIC ACID AND PROTEIN METABOLISM DURING THE MITOTIC CYCLE IN VICIA FABA The Journal of cell biology, 1961
- The incorporation of 3H-thymidine by developing root tip cellsExperimental Cell Research, 1960
- Basic Proteins of Plant Nuclei during Normal and Pathological Cell GrowthThe Journal of cell biology, 1959
- Nucleoprotein Changes in Plant Tumor GrowthThe Journal of cell biology, 1959
- The synthesis of nucleic acids and proteins in the nuclei of Tradescantia root tipsExperimental Cell Research, 1959
- Rhythmisches Kernwachstum während der mitotischen Interphase vonVicia fabaÖsterreichische botanische Zeitschrift, 1957
- CYTOLOGICAL STUDIES ON THE ANTIMETABOLITE ACTION OF 2,6-DIAMINOPURINE IN VICIA FABA ROOTSThe Journal of cell biology, 1955
- Untersuchungen an Interphasekernen des Wurzelmeristems von Vicia fabaChromosoma, 1953