Low frequency MFs increased inositol 1,4,5‐trisphosphate levels in the Jurkat cell line
- 13 February 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 359 (2-3), 151-154
- https://doi.org/10.1016/0014-5793(95)00031-4
Abstract
We have earlier reported that when a weak 50 Hz MF (magnetic field) was applied, the leukemic T-cell line Jurkat responded with intracellular calcium oscillations [Lindström, et al., J. Cell Physiol., 156 (1993) 395–398]. The result suggested that the MF interfered with the signal transduction, although neither target molecules nor molecular mechanisms are at present known. In this study we found that application of a MF to Jurkat cells resulted in significant increase of inositol 1,4,5-trisphosphate (IP3) levels. Chelation of intracellular calcium ions by BAPTA/AM, did not block the increase of IP3 induced by MF. This result implied that MF-induced Ca2+ oscillations were not due to direct stimulation of the Ca2+-dependent phospholipase C-γ1 (PLC-γ1)Keywords
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