Sucrose-induced ecological response of experimental dental plaques from caries-free and caries-susceptible Human volunteers
- 1 December 1981
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 34 (3), 662-675
- https://doi.org/10.1128/iai.34.3.662-675.1981
Abstract
Microbial succession, experimental cariogenicity and sucrose metabolism were examined in dental plaques which developed on sterile bovine enamel inserts in acrylic palatal appliances. The appliances were worn for a period of 14 days by 10 caries-free and 10 caries-susceptible human volunteers. Of 6 enamel inserts on each appliance 3 were exposed extraorally to 10% sucrose in 0.85% saline 6 times a day; 3 were exposed simultaneously to 0.85% saline as a control environment. The responses of the plaques to the high-sucrose environment in both caries status populations were compared. In all plaques, exposure to 10% sucrose stimulated the succession of Veillonella spp., Lactobacillus spp., Streptococcus salivarius and, to a lesser extent, S. mutans and a decline in levels of S. sanguis, Neisseria spp. and gram-negative anaerobic rods. Plaques from caries-free mouths, in contrast to those from caries-susceptible mouths, harbored higher levels of Veillonella spp., gram-negative anaerobic rods and Neisseria spp. and lower levels of Lactobacillus spp. Sucrose-exposed plaques from caries-free mouths also induced less enamel microhardness changes and formed less lactic acid from [14C]sucrose during a 60-min incubation at 37.degree. C than did comparable plaques from caries-susceptible mouths. The experiments revealed consistent differences in the ecological response to a cariogenic substrate environment in plaques from the 2 populations, with plaques from caries-free subjects exhibiting less cariogenic potential than those from caries-susceptible subjects.This publication has 36 references indexed in Scilit:
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