Chromosomal and cross‐mating evidence for two species within Anopheles (A.) coustani (Diptera: Culicidae)

Abstract

Examination of the polytene chromosomes of the fourth instar larvae provides evidence for the existence of two sibling species within the taxon Anopheles (Anopheles) coustani Laveran. Crosses between the two chromosomal types produces sterile male offspring. Since no classical morphological differences can be demonstrated in the adults, the probable existence of such characters in the larvae and pupae is discussed.