Comparison of Methods For Detecting Specific Igm Antibody in Infants With Congenital Rubella

Abstract

Serum specimens from 14 infants with congenital rubella were examined for specific IgM antibody by six different methods. IgM-containing fractions were separated either by sucrose density-gradient centrifugation or by gel filtration through Sephadex G-200, and were then tested by the indirect immunofluor-escence technique and by the haemagglutination-inhibition (HI) test (long- and short-incubation methods).