Different domains of the murine RNA polymerase I-specific termination factor mTTF-I serve distinct functions in transcription termination.
- 15 March 1995
- journal article
- Vol. 14 (6), 1248-56
Abstract
Termination of mouse ribosomal gene transcription by RNA polymerase I (Pol I) requires the specific interaction of a DNA binding protein, mTTF-I, with an 18 bp sequence element located downstream of the rRNA coding region. Here we describe the molecular cloning and functional characterization of the cDNA encoding this transcription termination factor. Recombinant mTTF-I binds specifically to the murine terminator elements and terminates Pol I transcription in a reconstituted in vitro system. Deletion analysis has defined a modular structure of mTTF-I comprising a dispensable N-terminal half, a large C-terminal DNA binding region and an internal domain which is required for transcription termination. Significantly, the C-terminal region of mTTF-I reveals striking homology to the DNA binding domains of the proto-oncogene c-Myb and the yeast transcription factor Reb1p. Site-directed mutagenesis of one of the tryptophan residues that is conserved in the homology region of c-Myb, Reb1p and mTTF-I abolishes specific DNA binding, a finding which underscores the functional relevance of these residues in DNA-protein interactions.This publication has 25 references indexed in Scilit:
- Has the breast cancer gene been found?Cell, 1994
- The POZ domain: a conserved protein-protein interaction motif.Genes & Development, 1994
- Transcription TerminationCritical Reviews in Biochemistry and Molecular Biology, 1993
- Limited proteolysis unmasks specific DNA-binding of the murine RNA polymerase I-specific transcription termination factor TTFIJournal of Molecular Biology, 1992
- The inducible transcription activator NF-κB: regulation by distinct protein subunitsBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1991
- Specific interaction of the murine transcription termination factor TTF I with class-I RNA polymerasesNature, 1990
- 3'-end formation of mouse pre-rRNA involves both transcription termination and a specific processing reaction.Genes & Development, 1989
- A transcription terminator located upstream of the mouse rDNA initiation site affects rRNA synthesisCell, 1986
- A repeated 18 bp sequence motif in the mouse rDNA spacer mediates binding of a nuclear factor and transcription terminationCell, 1986
- Transcription of mouse rDNA terminates downstream of the 3′ end of 28S RNA and involves interaction of factors with repeated sequences in the 3′ spacerCell, 1985