THE SITE OF ACIDIFICATION OF THE URINE IN THE DOG'S KIDNEY
- 1 June 1957
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 35 (6), 419-423
- https://doi.org/10.1139/o57-051
Abstract
Damage to the proximal tubules of one kidney in the dog resulted in a decrease in the acidity of the urine excreted by the damaged kidney. When ferric ammonium citrate and sodium ferrocyanide were injected intravenously into dogs excreting urines of pH 5.0 or less, Prussian blue was formed at the brush borders of the cells of the proximal tubules indicating an acidity of pH 3.5 or less. Injected Töpfer's reagent (range pH 2.9 to 4.0), and tropaeolin 00 (range pH 1.9 to 2.4) were seen in their acid forms at the same site. When a carbonic anhydrase inhibitor was injected into the renal artery of one kidney the above phenomena were not observed. When the urine pH was greater than 6.0 no Prussian blue was formed and the indicators listed were in their alkaline forms. In these animals injected bromothymol blue and bromocresol purple were seen in the lumina of the tubules in shades indicating a pH above 7.0 in the proximal tubules and about 6.0 to 6.5 in the distal tubules. It seems evident that, when dogs are excreting markedly acid urines, hydrogen ions are secreted in high concentration at the brush borders of the proximal convoluted tubules. On the other hand when the acidity of the urine is slight, acidification of the urine appears to take place in the distal tubule.Keywords
This publication has 7 references indexed in Scilit:
- THE ROLE OF ALDOSTERONE IN NORMAL HOMEOSTASIS AND IN CERTAIN DISEASE STATES1956
- A Modified Freezing-Drying Apparatus for TissuesStain Technology, 1951
- The site of hydrochloric acid production in the stomach as determined by indicatorsBiochemical Journal, 1950
- THE NATURE OF THE RENAL TUBULAR MECHANISM FOR ACIDIFYING THE URINEAmerican Journal of Physiology-Legacy Content, 1945
- THE SITE OF ACIDIFICATION OF URINE IN THE FROG'S AND RAT'S KIDNEYQuarterly Journal of Experimental Physiology and Cognate Medical Sciences, 1940
- RENAL FUNCTION AS AFFECTED BY EXPERIMENTAL UNILATERAL KIDNEY LESIONSThe Journal of Experimental Medicine, 1938
- THE SITE OF ACIDIFICATION OF THE URINE WITHIN THE RENAL TUBULE IN AMPHIBIAAmerican Journal of Physiology-Legacy Content, 1936