A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta
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- 1 October 2003
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 133 (2), 462-469
- https://doi.org/10.1104/pp.103.027979
Abstract
The growing expansion range of drug resistant parasitic nematode populations threatens the sustainability of ruminant farming worldwide. In this context, nutraceuticals, i.e. feed that would both fulfil dietary requirements while ensuring parasite control, would contribute to increase farming sustainability in developed and low resource settings. In this study, we characterized the anthelmintic potential of lupin seed extracts against major ruminant trichostrongylids, i.e. H. contortus and T. circumcincta. Our observations showed that total seed extracts from commercially available lupin varieties significantly inhibited larval migration. This anthelmintic effect was sustained on multidrug resistant field isolate and across parasite species and was mediated by the seed alkaloid content. Analytical chemistry revealed a set of four lupanine and sparteine-derivatives with anthelmintic activity and electrophysiology assays on recombinant nematode acetylcholine receptors suggested an antagonistic mode of action for lupin alkaloids. While commercial lupin seeds did not exert direct anthelmintic effect in H. contortus infected lupin-fed ewes and goats, it significantly dampened blood production losses suffered by goats. Lupin seed extracts hence provide a working basis for the development of novel anthelmintic compounds able to break drug resistance in the field, while they could be used to increase the resilience of infected dairy ruminants.Keywords
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