Modulation of nicotinic acetylcholine receptor channel by pH: A difference in pH sensitivity ofTorpedo and mouse receptors expressed inXenopus oocytes

Abstract

In this study, effects of pH on the ion channel function of nicotinic acetylcholine receptor (nAChR) fromTorpedo californica electroplax and mouse muscle BC3H-1 cells were investigated usingXenopus laevis oocytes injected within vitro synthesized RNA transcripts. The acetylcholine (ACh)-induced wholecell peak current responses and slow desensitization rates were measured by voltage-clamp. The ACh-induced peak currents ofTorpedo nAChRs were reversibly diminished when extracellular pH was reduced from 7.4 to 5.0 and increased at pH >7.4. This pH dependence had an apparent pK_a of 7.0. In contrast, the peak current of mouse muscle nAChRs were reversibly decreased at both acidic and alkaline pH's. This bell-shaped pH profile with a maximum at pH 7.4 had two apparent pK_a values of 5.6 and 9.2. The peak current responses of four mouse-Torpedo nAChR hybrids consisting of threeTorpedo subunits and one mouse nAChR subunit displayed similar pH profiles at acidic pH, with a pK_a of 6.0 to 6.5, which lay between the pK_a 5.6 for mouse and the pK_a 7.0 forTorpedo nAChRs. Two of these combinations,α_Mβ_Tγ_Tδ_T andα_Tβ_Tγ_Mδ_T, also had an alkaline pH dependence of the current response similar to that of mouse receptor, with a pK_a of 9.3 to 9.5. The slow desensitization rate ofTorpedo nAChRs increased at both acidic and alkaline pH's, with two pK_a values of 6.5 and 9.5, whereas that of mouse muscle receptors remained unchanged from pH 6.5 to pH 9.0 and increased at pH K_a of 4.7. Substitution of each subunit ofTorpedo nAChR with a mouse counterpart resulted in a pH dependence pattern (pK_a 6.0 to 6.4 and pK_a 9.1 to 9.3) similar to that ofTorpedo nAChR except the substitution with mouseβ subunit (pK_a 4.8 and pK_a 8.9), which appears to carry the characteristic acidic group determining the pH dependence of mouse nAChR desensitization. The apparent pK_a values obtained from the pH dependence studies of nAChR channel activation and desensitization suggest the involvement of different amino acid residues in determining channel functions ofTorpedo and mouse nAChRs. The groups with pK_a 7.0 and 6.5 onTorpedo nAChR can be tentatively identified as His residues, whereas those with pK_a 5.6 and 4.7 on mouse receptor as Glu or Asp residues. The alkaline pK_a of 8.9 to 9.5 may be Cys, Tyr, or Lys.