Deoxyribophosphate lyase activity of mammalian endonuclease VIII‐like proteins
Open Access
- 15 August 2006
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 580 (20), 4916-4922
- https://doi.org/10.1016/j.febslet.2006.08.011
Abstract
Base excision repair (BER) protects cells from nucleobase DNA damage. In eukaryotic BER, DNA glycosylases generate abasic sites, which are then converted to deoxyribo‐5′‐phosphate (dRP) and excised by a dRP lyase (dRPase) activity of DNA polymerase β (Polβ). Here, we demonstrate that NEIL1 and NEIL2, mammalian homologs of bacterial endonuclease VIII, excise dRP by β‐elimination with the efficiency similar to Polβ. DNA duplexes imitating BER intermediates after insertion of a single nucleotide were better substrates. NEIL1 and NEIL2 supplied dRPase activity in BER reconstituted with dRPase‐null Polβ. Our results suggest a role for NEILs as backup dRPases in mammalian cells.Keywords
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