H+ Fluxes in Excised Samanea Motor Tissue

Abstract
Homogeneous groups of cells were excised at regular intervals from opposing (extensor and flexor) motor tissue of S. saman (Jacq) Merrill maintained in white light for 34 h. H+ fluxes between the tissue and bathing solution were then monitored during 30 min of darkness. Flux rates in both cell types vary with circadian rhythms. Flexor cells secrete H+ to the medium during 2/3 of the circadian cycle and take up H+ during the remainder of the cycle, while extensor cells take up H+ from the medium during the entire cycle.