LH-Stimulable Adenylyl Cyclase Activity During the Ovulatory Cycle in Granulosa Cells of the Three Largest Follicles and the Postovulatory Follicle of the Domestic Hen (Gallus domesticus)
- 1 November 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 25 (4), 805-812
- https://doi.org/10.1095/biolreprod25.4.805
Abstract
The adenylyl cyclase (AC) activity in the granulosa (Gr) cells of the largest (F1), next largest (F2), third largest (F3), and postovulatory (POF) follicles throughout the ovulatory cycle of the domestic hen was measured. Granulosa cells were isolated at 24, 18, 12, 8, 6, 4, and 2 h before ovulation and basal and LH-stimulable AC activities were determined. There was a significant difference (P1 Gr at all times studied, with the most dramatic difference observed between 18 and 12 h before ovulation (P< 0.001) (18 h, 58.42 ± 4.72 vs 12 h, 105.13 ± 4.89 pmoles cAMP/min/mg protein). There was a transient decrease in basal and LH-stimulable AC activities after the LH surge, followed by a significant (P2 Gr cells were essentially the same as those of the F1 Gr except that the relative LH stimulation was less. In contrast, the pattern of AC activity of the F3 Gr cells differed from that of the F1 and F2 follicles. At all times assayed except 6 and 4 h before ovulation, the LH-stimulable activity was significantly greater (P<0.05) than basal values, which remained the same throughout the cycle. The LH-stimulable AC activity of the Gr cells of the POF was still significantly (P<0.001) elevated 2 h after ovulation, but declined to basal levels 4 h later. There was a significant increase in the basal AC activity of the POF at 24 h after ovulation.This publication has 23 references indexed in Scilit:
- AN HOMOLOGOUS RADIOIMMUNOASSAY FOR CHICKEN FOLLICLE-STIMULATING HORMONE: OBSERVATIONS ON THE OVULATORY CYCLEJournal of Endocrinology, 1977
- PRESENCE OF PROSTAGLANDIN-F (PGF) IN HEN FOLLICLES AND ITS PHYSIOLOGICAL-ROLE IN OVULATION AND OVIPOSITION1977
- The Porcine Ovarian Follicle: III. Development of Chorionic Gonadotropin Receptors Associated with Increase in Adenyl Cyclase Activity During Follicle MaturationEndocrinology, 1976
- Adenylyl Cyclase Activities in Ovarian Tissues. III. Regulation of Responsiveness to LH, FSH, and PGEX in the Prepubertal, Cycling, Pregnant, and Pseudopregnant RatEndocrinology, 1976
- Adenylyl Cyclase Activities in Ovarian Tissues. II. Regulation of Responsiveness to LH, FSH, and PGE1in the RabbitEndocrinology, 1976
- Adenylyl Cyclase Activities in Ovarian Tissues. I. Homogenization and Conditions of Assay in Graafian Follicles and Corpora Lutea of Rabbits, Rats, and Pigs: Regulation by ATP, and Some Comparative PropertiesEndocrinology, 1976
- Refractoriness of ovarian adenylate cyclase to continued hormonal stimulationBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- HORMONAL CONTROL OF THE HEN'S OVULATION CYCLEJournal of Endocrinology, 1967
- Changes in Hypophysial and Plasma LH Levels During the Laying Cycle of the HenEndocrinology, 1965
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951