Effects of excessive selenium supplementation to diet contaminated with deoxynivalenol on blood phagocytic activity and antioxidative status of broilers
- 6 November 2009
- journal article
- research article
- Published by Wiley in Journal of Animal Physiology and Animal Nutrition
- Vol. 93 (6), 695-702
- https://doi.org/10.1111/j.1439-0396.2008.00857.x
Abstract
This study examined the effects of excessive dietary supplementation with organic selenium on phagocytic activity and antioxidative status of chickens for fattening fed diet contaminated with deoxynivalenol (DON). Sixty chickens of Ross 308 hybrids were at day of hatching divided into four groups with 15 birds in each. The background DON dietary levels in both negative and positive control groups were 0.2 mg/kg. The complete feed for positive control group was supplemented with Se dose 1 mg/kg in the form of Se-yeast. Group 3 was fed diet with DON level 3 mg/kg while diet for group 4 combined DON level 3 mg/kg with a excessive supplement of Se-yeast (Se dose 1 mg/kg). After 6 weeks of dietary intake, six randomly-chosen chickens from each group were sampled. Feeding of contaminated diet resulted in significantly reduced blood phagocytic activity (19.5 ± 1.1% in the negative control vs. 12.8 ± 0.8% in the DON-treated group, p < 0.05). Se-yeast supplemented to the DON contaminated diet prevented suppression of phagocytic activity. Dietary intake of DON at levels 3 mg/kg did not influence the plasma α-tocopherol level while excessive dietary Se dose reduced it in both Se supplemented groups. Neither the birds of DON-treated group nor the birds from group 4 with DON and Se-yeast showed any response in plasma γ-glutamyltransferase (GGT) activity. Subtoxic dietary level of DON significantly increased the activity of glutathione peroxidase (GPx) in the duodenal mucosa, while additional Se supplementation prevented such a response to mycotoxin. On the other hand, both Se supplemented groups showed significantly elevated GPx activities in blood, liver and kidney, (p < 0.05). The results suggest a potential ability of excessive supplementation of organic selenium to prevent the blood phagocytic activity suppression and changes in GPx activity in duodenal tissue induced in broilers by subtoxic dietary levels of DON.Keywords
This publication has 26 references indexed in Scilit:
- Influence of deoxynivalenol on NF-κB activation and IL-8 secretion in human intestinal Caco-2 cellsToxicology Letters, 2008
- Influence of deoxynivalenol on the D‐glucose transport across the isolated epithelium of different intestinal segments of laying hensJournal of Animal Physiology and Animal Nutrition, 2007
- Influence of dietary selenium and vitamin E on quality of vealMeat Science, 2007
- Effects of selenium depletion and selenium repletion by choice feeding on selenium status of young and old laying hensPhysiology & Behavior, 2006
- The efficacy of a modified aluminosilicate as a detoxifying agent in Fusarium toxin contaminated maize containing diets for pigletsJournal of Animal Physiology and Animal Nutrition, 2005
- Activation of Transcription Factor Nrf-2 and Its Downstream Targets in Response to Moloney Murine Leukemia Virusts1-Induced Thiol Depletion and Oxidative Stress in AstrocytesJournal of Virology, 2004
- Reactive Oxygen Species Act at both TGF-β-Dependent and -Independent Steps during Induction of Apoptosis of Transformed Cells by Normal CellsExperimental Cell Research, 1996
- Tissue Distribution and Excretion of Radioactivity following Administration of 14C-Labeled Deoxynivalenol to White Leghorn HensToxicological Sciences, 1986
- Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient cattleLife Sciences, 1985
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976