Effect of anti-inflammatory medication on monocyte response to titanium particles

Abstract

Cytokines produced by macrophages in the periprosthetic membranes surrounding joint replacements have been implicated as causal agents in osteolysis and prosthetic loosening. The present study characterizes the response of human peripheral blood monocytes to titanium particles. Monocytes were obtained from donated blood and were cultured in the presence of different‐sized titanium particles. Exposure to titanium‐aluminum‐vanadium particles significantly changed the release of tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6), and interleukin‐1 (IL‐1), whereas there was no significant effect on the release of prostaglandin E₂ (PGE₂). When monocytes were cultured with particles, the titanium alloy particles induced significantly more release of TNF‐α and less IL‐1 secretion. Ciprofloxacin inhibited production of TNF‐α, IL‐6, IL‐1, and PGE₂ in human monocytes exposed to titanium particles. In contrast to ciprofloxacin, indomethacin was not a potent inhibitor of TNF‐α production but potentiated IL‐6 production in titanium‐stimulated monocytes. Indomethacin had no effect on the production of IL‐1 and was a potent inhibitor of PGE₂ production in titanium‐stimulated monocytes. Pentoxifylline had an inhibitor effect on TNF‐α production in titanium‐stimulated monocytes. Pentoxifylline potentiated IL‐6 and IL‐1 production in monocytes exposed to titanium particles and had a biphasic effect on the PGE₂ production. The results of this study support our hypothesis that human monocytes release bone resorption mediators after in vitro exposure to TiAlV alloy particles. The results also demonstrate the differences of bone‐resorbing mediators in response to different wear particle size. The pharmacologic agents (ciprofloxacin, pentoxifylline, and indomethacin) that can modulate the release of bone resorbing mediators such as PGE₂, TNF‐α, IL‐1, and IL‐6 release from human monocytes. The results help to elucidate the differences in cellular response to wear particles but may not be directly transposed to the human situation. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 52, 509–516, 2000.