Kinetic studies on the binding of cyanide to oxygenated cytochrome c oxidase
- 1 May 1976
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 155 (2), 453-455
- https://doi.org/10.1042/bj1550453
Abstract
The reaction of cyanide with oxygenated cytochrome c oxidase was followed by means of flow-flash techniques. The oxygenated form, produced after photolysis of the partially reduced CO complex in the presence of cyanide and O2, shows cyanide-binding properties distinct from those of both the oxidized and the reduced forms of the protein. The binding is a single process (k = 22M-1-S-1) linearly dependent on cyanide concentration to as high as 75 mM. It is suggested that the oxygenated form is a conformational variant of the oxidized protein.This publication has 11 references indexed in Scilit:
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