Prostaglandin E2 Production in 3T3 Cells Transformed by Polyoma Virus Raises the Intracellular Adenosine 3′: 5′‐Monophosphate Levels

Abstract

High cellular cyclic[c]AMP levels were found in a polyoma-virus-transformed 3T3 [mouse embryo] fibroblast line, which produces comparatively high prostaglandin [PG] E2 concentrations. PG E2 and cAMP increased with time during 6 days of growth. Both effects were prevented by 3 different cyclo-oxygenase inhibitors. Addition of PG E2, at a concentration which would have been synthesized in the absence of inhibitor, reversed the effect of indomethacin, 1 of the cyclo-oxygenase inhibitors, on cAMP. Endogenous PG E2 production in these transformed cells apparently influences their cellular cAMP levels.