Ultraviolet-induced autofluorescence of arbuscular mycorrhizal root infections: an alternative to clearing and staining methods for assessing infections
- 1 March 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 28 (3), 351-355
- https://doi.org/10.1139/m82-052
Abstract
Arbuscules in vesicular–arbuscular mycorrhizal (VAM) infections in untreated roots autofluoresced when viewed with an epifluorescence microscope. Ultraviolet-induced fluorescence was observed with the same filtering system used to detect fluorescein staining. Plant roots were examined from five plant families, grown under a variety of conditions, and infected with several species of VAM fungi. In every case, autofluorescent arbuscules were observed. Vesicles, spores, and hyphae did not autofluoresce under these same conditions. Fluorescence was not observed in ectomycorrhizae or pathogenic fungal infections. Cut mycorrhizal root segments from greenhouse- and field-grown plants were evaluated for infection using ultraviolet light and clearing and staining. No significant differences were found in the percent VAM infection as determined by the two methods on the same root segments. Applications and considerations in the use of the ultraviolet method for measuring infections as well as some preliminary observations on using fluorescein diacetate are discussed.This publication has 8 references indexed in Scilit:
- Autofluorescence in oats infected by powdery mildewTransactions of the British Mycological Society, 1981
- AN EVALUATION OF TECHNIQUES FOR MEASURING VESICULAR ARBUSCULAR MYCORRHIZAL INFECTION IN ROOTSNew Phytologist, 1980
- Development of a vesicular-arbuscular mycorrhiza in bean rootsCanadian Journal of Botany, 1979
- INCORPORATION OF 14C‐LABBLLED SUBSTRATES BY UNINFETED AND VA MYCORRHIZAL ROOTS OF ONIONNew Phytologist, 1979
- Ultrastructural Cytochemistry of the Host-Fungus Interfaces in the Endornycorrhizal Association Glomus mosseae / Allium cepaZeitschrift für Pflanzenphysiologie, 1979
- COLORIMETRIC QUANTIFICATION OF VESICULAR‐ARBUSCULAR MYCORRHIZAL INFECTION IN ONIONNew Phytologist, 1977
- Vital staining of fungi in pure cultures and in soil with fluorescein diacetateSoil Biology and Biochemistry, 1977
- TRANSLOCATION AND TRANSFER OF NUTRIENTS IN VESICULAR–ARBUSCULAR MYCORRHIZASNew Phytologist, 1976