PREPARATION OF THROMBOKINASE FROM BOVINE PLASMA
Open Access
- 20 March 1959
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 42 (4), 665-676
- https://doi.org/10.1085/jgp.42.4.665
Abstract
Thrombokinase is prepared from bovine plasma by a procedure involving: treatment with diatomaceous silica, adsorption on barium sulfate, flowing elution with two successive phosphate buffers, ammonium sulfate fractionation, "spontaneous" activation in concentrated solution, and isoelectric precipitation. The yield of nitrogen is 0.002 per cent, corresponding to 1.2 mg. protein per liter of plasma.Keywords
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