Purification of Terminal Deoxynucleotidyl Transferase from Pig Thymus: Identification of 42,000 and 57,000 Dalton Species1
- 1 October 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 90 (5), 1421-1427
- https://doi.org/10.1093/oxfordjournals.jbchem.a133608
Abstract
Terminal deoxynucleotidyl transferase [EC 2.7.7.3 1] has been purified 4,365-fold from pig thymus. It was further separated into two molecular forms of 57,000 and 45,000 daltons by Sephadex G-100 gel-filtration. The former sedimented at 4.2S through a sucrose gradient, while the latter sedimented at 3.6S. By sodium dodecyl sulfate-polyacrylamide gel-electrophoresis, their molecular weights were estimated as 57,000 and 42,000 daltons, respectively. Thus, the large and small pig terminal deoxynucleotidyl transferases both consist of a single polypeptide of 57,000 or 42,000 daltons and have no subunit structure. These two forms were indistinguishable in antigenicity as examined by a neutralization assay with an anticalf terminal deoxynucleotidyl transferase antibody. The enzymatic properties of 42,000-dalton terminal deoxynucleotidyl transferase from pig thymus were very similar to those of the calf enzyme, which has a two-subunit structure.This publication has 12 references indexed in Scilit:
- TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE-ACTIVITY IN THE BLASTIC CELLS OF ACUTE LYMPHOBLASTIC-LEUKEMIA OF CHILDHOOD AND ADOLESCENTS - A PREDICTOR OF RESPONSIVENESS TO VINCRISTINE-PREDNISOLONE THERAPY1980
- Biosynthesis of murine terminal deoxynucleotidyltransferase.Journal of Biological Chemistry, 1980
- Purification of a high molecular weight human terminal deoxynucleotidyl transferase.Journal of Biological Chemistry, 1979
- Relation of “lymphoid” phenotype and response to chemotherapy incorporating vincristine-prednisolone in the acute phase of Ph1 positive leukemiaCancer, 1979
- Terminal deoxynucleotidyl transferase in the diagnosis of leukemia and malignant lymphomaAmerican Journal Of Medicine, 1978
- TERMINAL DEOXYNUCLEOTIDYL-TRANSFERASE LEVELS AND MEMBRANE PHENOTYPES IN DIAGNOSIS OF ACUTE LEUKÆMIAThe Lancet, 1977
- Terminal Deoxynucleotidyl Transferase Activity in Malignant LymphomasNew England Journal of Medicine, 1977
- DEOXYNUCLEOTIDE-POLYMERIZING ENZYMES OF CALF THYMUS GLAND .2. PROPERTIES OF TERMINAL DEOXYNUCLEOTIDYLTRANSFERASE1967
- Oligodeoxyribonucleotide-primed Reactions Catalyzed by Calf Thymus PolymeraseJournal of Biological Chemistry, 1962
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951