Changes in surface ATPase of rat spermatozoa in transit from the caput to the cauda epididymidis

Abstract

Rat spermatozoa from the cauda epididymidis had a lower activity of the surface ATPase than the spermatozoa from the caput region. The enzyme from spermatozoa of both regions had the same Km for ATP of 5 .times. 10-4 M. It was partly inhibited by ouabain and fluoride, but strongly inhibited by Cu2+, Zn2+, p-chloromercuribenzoate, 8-anilino-1-naphthalenesulfonate Triton X-100, Lubrol-PX, urea, guanidine hydrochloride, sodium dodecyl sulfate and glycerylphosphorylcholine. The enzyme of the spermatozoa from the cauda epididymidis was more sensitive to inhibition by ouabain and fluoride but less sensitive to inhibition by Cu2+ than that of the cells from the caput region. The Arrhenius plot of the temperature dependence of enzymatic activity varied for the cells from the caput and cauda epididymidis. The differences in the enzyme properties of spermatozoa from the 2 regions of the epididymis suggested that the decline in the activity during epididymal maturation may reflect changes in the lipids and sulfydryl groups of the sperm membrane.