Methods For Calculating the Internal Temperature of Capillary Columns During Capillary Electrophoresis

Abstract

The internal temperature, the buffer viscosity and the efficiency of heat removal from a silica capillary can be calculated by measuring the differential electroosmostic mobility at a low voltage and a high voltage. the calculated temperature is plotted vs the power generated by the electrophoretic instrument and yields a linear relationship when the power is below 3.0 W. the temperature can also be calculated using the conductivity of the solution. the two methods provide the same temperature, which compares well with literature values. A rule of thumb for a quick calculation of internal temperatures is that a power of 0.1 W