A complete separation of dimethylaminoazobenzenesulphonyl-amino acids. Amino acid analysis with low nanogram amounts of polypeptide with dimethylaminoazobenzenesulphonyl chloride
- 1 June 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 203 (3), 803-806
- https://doi.org/10.1042/bj2030803
Abstract
A chromatographical system has been developed to give a complete baseline separation of all dimethylaminoazobenzenesulphonyl (DABS)-amino acids for high-sensitivity amino acid analysis [Chang, Knecht & Braun (1981) Biochem. J. 199, 547-556]. The system, which uses a Merck RP-18 column with phosphate buffer (12 mM, pH 6.5)/acetonitrile mixture, allows reliable analysis of DABS-amino acids at the 1-2 pmol level. The accuracy of this new system is demonstrated by the composition analysis of two immunoglobulin light chains (214 amino acid residues) with differences at only three amino acid residue positions.This publication has 3 references indexed in Scilit:
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- STRUCTURE OF THE ELASTASE-CATHEPSIN-G INHIBITOR OF THE LEECH HIRUDO-MEDICINALIS1980
- Chromophoric labeling of amino acids with 4-dimethylaminoazobenzene-4'-sulfonyl chlorideAnalytical Chemistry, 1975