Mesoderm-inducing factors and Spemann’s organiser phenomenon in amphibian development
Open Access
- 1 October 1989
- journal article
- research article
- Published by The Company of Biologists in Development
- Vol. 107 (2), 229-241
- https://doi.org/10.1242/dev.107.2.229
Abstract
Certain proteins from ‘growth factor’ families can initiate mesodermal development in animal cap cells of the amphibian blastula. Cells that are in early stages of their response to one such factor, XTC-MIF (Smith et al. 1988), initiate the formation of a new axial body plan when grafted to the ventral marginal zone of a similarly aged host embryo (Cooke et al. 1987). This replicates the natural control of this phase of development by the dorsal blastoporal lip when similarly grafted; the classical ‘organiser’ phenomenon. I have explored systematically the effect, upon the outcome of this pattern formation using defined inducing factors, of varying graft size, XTC-MIF concentration to which graft cells were exposed, length of exposure before grafting, and host age. The ‘mesodermal organiser’ status, evoked by the factor, appears to be stable, and the variables most influencing the degree of completeness and orderliness of second patterns are graft size and factor concentration. Inappropriately large grafts are not effective. A Xenopus basic fibroblast growth factor homologue, present in the embryo and known to be a strong inducer but of mesoderm with a different character from that induced by XTC-MIF, produced no episode of pattern formation at all when tested in the procedure described in this paper. Organiser status of grafts that have been exposed to mixtures of the two factors is set entirely by the supplied XTC-MIF concentration. Lineage labelling of these grafts, and of classical dorsal lip grafts, reveals closely similar though not identical patterns of contribution to the new structure within the host. Implications of the results for the normal mechanism of body pattern formation are discussed.This publication has 39 references indexed in Scilit:
- Gastrulation and larval pattern in Xenopus after blastocoelic injection of a Xenopus-derived inducing factor: Experiments testing models for the normal organization of mesodermDevelopmental Biology, 1989
- A gradient of bicoid protein in Drosophila embryosCell, 1988
- Mesoderm induction in early Xenopus embryos by heparin-binding growth factorsNature, 1987
- Functional gap junctions are not required for muscle gene activation by induction in Xenopus embryos.The Journal of cell biology, 1987
- High-frequency twinning of Xenopus laevis embryos from eggs centrifuged before first cleavageDevelopmental Biology, 1986
- Activation of muscle-specific actin genes in xenopus development by an induction between animal and vegetal cells of a blastulaCell, 1985
- Early cellular interactions promote embryonic axis formation in Xenopus laevisDevelopmental Biology, 1984
- Axis determination in eggs of Xenopus laevis: A critical period before first cleavage, identified by the common effects of cold, pressure and ultraviolet irradiationDevelopmental Biology, 1983
- Scale of body pattern adjusts to available cell number in amphibian embryosNature, 1981
- Über den Anteil von Implantat und Wirtskeim an der Orientierung und Beschaffenheit der induzierten EmbryonalanlageWilhelm Roux' Archiv für Entwicklungsmechanik der Organismen, 1931