Covalent attachment of proteins to Spheron by means of benzoquinone

Abstract

The dependence of the amount and the activity of coupled chymotrypsin and trypsin on the activation time and pH, coupling time and pH, enzyme concentration in the reaction mixture and the molar ratio CaCl₂/trypsin concentration were investigated for case where enzymes are covalentlybound to the hydroxyalkyl methacrylate gel (Spheron) by means of benzoquinone.