THE Ibc PROTEIN FRACTION OF GROUP B STREPTOCOCCI: CHARACTERIZATION OF PROTEIN ANTIGENS EXTRACTED BY HC1
- 1 March 1981
- journal article
- research article
- Published by Wiley in Acta Pathologica Microbiologica Scandinavica Section B Microbiology
- Vol. 89B (1-6), 205-209
- https://doi.org/10.1111/j.1699-0463.1981.tb00177_89b.x
Abstract
The Ibc protein fraction of group B streptococci was prepared by HCl extraction of the type Ic strains A909 and 335. The fraction from strain A909 contained 2 protein antigens (.alpha.A909 and .beta.A909) that could be separated by ion-exchange chromatography and isoelectric focusing. The 335 extract contained the .alpha. (.alpha.335) antigen, but not the .beta. antigen. The .alpha.335 antigen was purified by similar procedures. The .beta.A909 antigen had a MW of several hundred thousands, was immunogenic in rabbits and dissociated into several polypeptides on SDS-PAGE [sodium dodecyl sulfate polyaccylamide gel electrophoresis]. Polypeptides with sub-unit MW corresponding to 70,000; 45,000; and 23,000 daltons showed antigenic activity. The .alpha.335 antigen had a MW of .apprx. 75,000 daltons as judged from gel filtration and SDS-PAGE. The antigen was immunogenic in rabbits. In contrast, the .alpha.A909 antigen showed neither protein lines on SDS-PAGE, nor immunogenicity in rabbits. However, the 2 .alpha. antigens showed serological crossreactivity in tests with the anti-.alpha.335 serum.Keywords
This publication has 18 references indexed in Scilit:
- Complete And Incomplete Ibc Protein Fraction in Group B StreptococciActa Pathologica Microbiologica Scandinavica Section B Microbiology, 1979
- TYPE CLASSIFICATION OF GROUP B STREPTOCOCCI BY THE FLUORESCENT ANTIBODY TESTActa Pathologica Microbiologica Scandinavica Section B Microbiology, 1977
- Multiple mouse-protective antibodies directed against group B streptococci. Special reference to antibodies effective against protein antigens.The Journal of Experimental Medicine, 1975
- General Remarks on Principles, Equipment, Reagents and ProceduresScandinavian Journal of Immunology, 1973
- Distribution by Serological Type of Group B Streptococci Isolated from a Variety of Clinical Material over a Five-Year Period (with Special Reference to Neonatal Sepsis and Meningitis)Infection and Immunity, 1973
- The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1969
- Quantitative Determination of Carbohydrates With Dreywood's Anthrone ReagentScience, 1948
- TWO SEROLOGICAL TYPES OF GROUP B HEMOLYTIC STREPTOCOCCI WITH RELATED, BUT NOT IDENTICAL, TYPE-SPECIFIC SUBSTANCESThe Journal of Experimental Medicine, 1938
- A SEROLOGICAL DIFFERENTIATION OF SPECIFIC TYPES OF BOVINE HEMOLYTIC STREPTOCOCCI (GROUP B)The Journal of Experimental Medicine, 1934