Oxygen-Linked Hydrogen Ion Binding of Human Hemoglobin. Effects of Carbon Dioxide and 2,3-Diphosphoglycerate

Abstract

The Bohr coefficient, b, and the Haldane coefficient, h, were measured simultaneously in erythrolysates in the presence and absence of CO2and 2,3-diphosphoglycerate. We found b—h=—0.04 (—0.023 to —0.054) although b—h=—0 according to the linkage equation. The cause of the observed difference seems to be that his measured as the mean value for oxygen saturation fractions (zHbO2) between 0 and 1, while brefers to a zHbO2of 0.54. Apparently b(and h) varies with zHbO2and reaches a numerical maximum at an intermediate zHbO2value.