Processing of Bacillus subtilis succinate dehydrogenase and cytochrome &‐558 polypeptides
- 23 March 1987
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 213 (2), 385-390
- https://doi.org/10.1016/0014-5793(87)81527-2
Abstract
The DNA sequence of the Bacillus subtilis sdh operon coding for the two succinate dehydrogenase subunits and cytochrome b-558 (the membrane anchor protein) has recently been established. We have now determined the extent of N-terminal processing of each polypeptide by radiosequence analysis. At the same time, direct evidence for the correctness of the predicted reading frames has been obtained. The cytochrome showed a ragged N-terminus, with forms lacking one residue, and is inserted across the membrane without an N-terminal leader-peptide. Covalently bound flavin was not detectable in B. subtilis succinate dehydrogenase expressed in Escherichia coli despite normal N-terminal processing of the apoprotein. This provides an explanation to why the succinate dehydrogenase synthesized in E. coli is not functional and demonstrates that host-specific factors regulate the coenzyme attachmentKeywords
This publication has 28 references indexed in Scilit:
- Bacillus subtilis mutant succinate dehydrogenase lacking covalently bound flavin: identification of the primary defect and studies on the iron-sulfur clusters in mutated and wild-type enzymeBiochemistry, 1986
- [38] Molecular properties, genetics, and biosynthesis of Bacillus subtilis succinate dehydrogenase complexMethods in Enzymology, 1986
- In vivo and in vitro expression of the 6‐hydroxy‐D‐nicotine oxidase gene of Arthrobacter oxidans, cloned into Escherichia coli, as an enzymatically active, covalently flavinylated polypeptideFEBS Letters, 1985
- Multiple Mechanisms of Protein Insertion into and Across MembranesScience, 1985
- The prosthetic groups of succinate dehydrogenase: 30 years from discovery to identificationFEBS Letters, 1985
- THE MITOCHONDRIAL ELECTRON TRANSPORT AND OXIDATIVE PHOSPHORYLATION SYSTEMAnnual Review of Biochemistry, 1985
- Succinate Dhydrogenase Mutants of Bacillus Suybtilis Lacking Covalently Bound Flavin in the Flavoprotien SubunitEuropean Journal of Biochemistry, 1983
- Sequence of the lactose permease geneNature, 1980
- Rapid analysis of amino acid phenylthiohydantoins by high-performance liquid chromatographyAnalytical Biochemistry, 1977
- [13] Bacterial MembranesPublished by Elsevier ,1971