Enzymatic transamination: The synthesis of α,β,β-Trideutero-L-glutamic acid and α-Dentero-L-glutamic acid

Abstract

α,β,β-Trideutero-L-glutamic acid has been synthesized by incubating L- glutamic acid in D2O at pD 7.8 in the presence of pig-heart glutamate- oxaloacetate aminotransferase and catalytic amounts of pyridoxal 5?- phosphate and oxaloacetic acid. However, when L-glutamic acid is incubated under similar conditions with pig-heart glutamate-pyruvate aminotransferase, in the presence of catalytic amounts of pyridoxal 5?- phosphate and pyruvic acid, α-deutero-L-glutamic acid is obtained.